Multiple-input multiple-output (MIMO) wireless technology in combination with orthogonal frequency division multiplexing (MIMO OFDM) is an attractive air-interface solution for next-generation wireless local area networks (WLANs), wireless metropolitan area networks (WMANs), and fourth-generation mobile cellular wireless systems. In this paper, one multiuser MIMO OFDM systems with TDD/TDMA was proposed for next-generation wireless mobile communications, i.e., TDD/TDMA 4G, which can avoid or alleviate the specific limitations of existing techniques designed for multiuser MIMO OFDM systems in broadband wireless mobile channel scenarios, i.e., bad performance and extreme complexity of multiuser detectors for rank-deficient multiuser MIMO OFDM systems with CDMA as access modes, extreme challenges of spatial MIMO channel estimators in rank-deficient MIMO OFDM systems, and exponential growth complexity of optimal sub-carrier allocations for OFDMA-based MIMO OFDM systems. Furthermore, inspired from the Steiner channel estimation method in multi-user CDMA uplink wireless channels, we proposed a new design scheme of training sequence in time domain to conduct channel estimation. Training sequences of different transmit antennas can be simply obtained by truncating the circular extension of one basic training sequence, and the pilot matrix assembled by these training sequences is one circular matrix with good reversibility. A novel eigenmode transmission was also given in this paper, and data symbols encoded by space-time codes can be steered to these eigenmodes similar to MIMO wireless communication systems with single-carrier transmission. At the same time" an improved water-filling scheme was also described for determining the optimal transmit powers for orthogonal eigenmodes. The classical water-filling strategy is firstly adopted to determine the optimal power allocation and correspondent bit numbers for every eigenmode, followed by a residual power reallocation 123 290 L. Zhaogan et al. to further determine the additional bit numbers carried by every eigenmode. Compared with classical water-filling schemes, it can also obtain larger throughputs via residual power allocation. At last, three typical implementation schemes of multiuser MIMO OFDM with TDMA, CDMA and OFDMA, i.e., TDD/TDMA 4G, VSF-OFCDM and FuTURE B3G TDD, were tested by numerical simulations. Results indicated that the proposed multiuser MIMO OFDM system schemes with TDD/TDMA, i.e., TDD/TDMA 4G, can achieve comparable system performance and throughputs with low complexity and radio resource overhead to that of DoCoMo MIMO VSF-OFCDM and FuTURE B3G TDD.