Background: Chronic kidney failure (CRF) is characterized by a progressive loss of functional status over weeks or months, which may result in one of the illness's recognized consequences, such as cardiovascular disease, chest pain, or anemia. CRF has been generally understood as a worldwide public health problem and a big factor to death and morbidity during the previous decade. Methods: Determination of zinc and copper, urea, creatinine, total protein, globulin, alanine aminotransferase (ALT), albumin, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in (CRF) Iraqi patients. This study included (40) patients and (38) healthy subjects as control. Samples were collected from January to April (2022). The biochemical parameters were determined using spectrophotometrically, but zinc and copper were determined by atomic absorption spectrophotometer. Results: Copper, urea, creatinine, ALP, AST, ALT, and LDH were significantly higher in CRF patients, whereas zinc, total protein, albumin, and globulin were lower in CRF patients as compared with control. The research also concerned study the relation between sex (male and female) and biochemical parameters which showed significant differences in comparison male of patients with males of control, yet globulin showed nonsignificant differences. While patients and control females showed nonsignificant differences. Conclusions: LDH in CRF patients might be used as a biomarker to detect renal dysfunction in dialysis patients at an early point, and blood serum Zn deficit in CRF. Furthermore, a relationship among Zn and albumin amounts was discovered. As a result, Zn and copper in CRF, particularly hypoalbuminemia, must be frequently evaluated and adjusted.