The complete mitochondrial genome of the cotton-melon aphid, Aphis gossypii Glover, was sequenced using a combination of high-throughput sequencing, traditional PCR amplification, and Sanger sequencing. The genome is 15,869 bp in length, and contains 37 typical coding genes, one non-coding AT-rich region, and a repeat region found exclusively in aphids. The base composition of the genome is A (45.4%), T (38.3%), C (10.4%), and G (5.9%). All protein coding genes start with a typical ATN initiation codon; all genes use the standard termination codon (TAA) except ND4 that ends with a single TA.
The study was conducted to evaluate the effect of various botanical products on population of sucking complex on cotton crop. The study was conducted at the experimental area of Entomology section, Agriculture Research Institute, Tando Jam during the Kharif Season-2004. Five bio-pesticides i.e. neem oil (500ml/acre), cooking oil (750 ml/acre), linseed oil (750ml/acre), hing (290gm/acre) and cotton oil (750ml/acre) were applied twice against sucking complex (jassid, thrip and whitefly) and compared with an untreated control. It was observed that the efficacy of different bio-pesticides against sucking complex (jassid, whitefly and thrips) varied significantly. All products reduced pest population during both sprays. On an average, neem oil (63.27%) and cotton oil (62.01%) were found to be superior in reducing sucking pest, followed by hing (58.25%), cooking oil (57.18%) and linseed oil (55.24%) respectively.
Nitrogen (N) fertilization at higher rates enhances the yield of crop plants; however, overuse of N in cultivation of crop not only decreased Nitrogen Use Efficiency of crop plants but caused severe environmental pollution. Hence, the optimum use of N is perquisite for sustainable development of Agriculture. This study was carried out during 2016, to evaluate the effect of various nitrogen applications on the economic performance of muskmelon. This research work was laid out at experimental site of Horticulture orchard SAU Tandojam with three replications in RCBD. The growth and yield performance of muskmelon was assessed by using six nitrogen (N) levels viz; 0, 30, 60, 90, 120 and 150 kg ha-1. Two varieties including Chandny and golden tumbro were used in the current study.The result showed that effect of different nitrogen doses on the economic important parameters of muskmelon was significant (P
A laboratory study was conducted to evaluate the influence of different temperatures on life stages of Chrysoperla carnea (Stephens) on frozen eggs of Sitotroga crealella. The study was conducted under laboratory conditions at Department of Plant Protection, SAU, Tandojam Sindh, Pakistan during 2013-14. The result revealed that the maximum hatching 88 % of eggs was recorded at 28 ºC followed by 25, 31, 22, 34 and 37 ºC. The highest mortality (dx) was recorded in first instar at 34 ºC followed by at 22, 31, 37, 25 and 28 ºC, whereas, minimum (dx) was recorded in third instar and pupal stages as well. The highest and lowest apparent mortality (100qx) was observed in the first instar and egg stages at 37 ºC. The data further depicted that the highest survival fraction (Sx) was recorded as (0.98) in second instar, third instar and pupa at 28 ºC, whereas, the lowest (Sx) was observed as (0.13) in the first instar at 37 ºC. The maximum indispensable mortality (IM) was 42 in egg stage at 37 ºC and lowest 1.0 in pupal stage at all temperature regimes. The number of the surviving at the beginning of the stage (lx) was highest 39 adults emerged at 28 ºC followed by 31, 24, 20 and 5 adults emerged at 25, 31, 22 and 34 ºC, respectively. On the other hand, minimum total generation mortality (K) was recorded as 0.11 at 28 ºC followed by 0.21, 0.32, 0.40, and 1.00 at 25, 31, 22 and 34 ºC, respectively. The minimum duration from egg to adult emergence was 12.0 days at 34 ºC and maximum 23.5 days at 22 ºC. There was significant difference between the duration and treatments (P<0.05). It is concluded that the maximum mortality was recorded at 37 ºC in egg and first instar stages, no any stage was survived after second instar. The temperature ranges from 25 to 31 ºC have been proved suitable for the development of C. carnea.. However, 1 st and 2 nd instar survived for short period at 37 ºC only.
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