Ikan asap pinekuhe merupakan suatu produk olahan hasil perikanan yang menjadi ciri khas Kabupaten Kepulauan Sangihe. Produk ini hanya bisa didapatkan di sekitaran Kepulauan Sangihe sehingga menjadi ciri khas daerah. Tujuan dari penelitian ini yaitu untuk mengetahui karakteristik mutu kimia dari ikan asap pinekuhe. Penelitian ini dilakukan menggunakan Rancangan Acak Kelompok (RAK) dengan 3 kali ulangan, dengan variabel pengamatan sebagai berikut; (A) Sampel dari Kecamatan Tahuna, (B) Sampel dari Kecamatan Tabukan Tengah, (C) Sampel dari Kecamatan Manganitu. (D)Sampel dari Kecamatan Tabukan Utara. Kesimpulan dari penelitian ini adalah ikan asap pinekuhe memiliki mutu kimia yang sesuai dengan Standar Nasional Indonesia (SNI) dengan tidak ada perbedaan signifikan antara tiap-tiap perlakuan/lokasi produksi. Rincian sebagai berikut; Kadar air terendah yaitu 55% dan tertinggi 59%, Kadar NaCl terendah yaitu 0.76% dan tertinggi 1.03%, kadar histamine terendah yaitu 40.9 mg/kg dan tertinggi yaitu 55.1%, Kadar Total Volatil Base Nitrogen (TVB-N) terendah 19.7 mgN/100g dan tertinggi 29.7 mgN/100g.
Virgin Coconut Oil (VCO) yang merupakan salah satu produk olahan kelapa yang memiliki banyak manfaat kesehatan sulit dikonsumsi secara langsung karena rasanya yang berminyak (oily). Oleh karena itu, untuk membuatnya enak dikonsumsi, VCO dibuat dalam bentuk minuman emulsi. Pada penelitian ini, minuman emulsi VCO ditambahkan dengan jahe merah dan sereh yang bertujuan untuk menyelidiki pengaruh penambahan jahe merah dan sereh pada karakteristik fisik, kimia, aktivitas antioksidan, dan tingkat kesukaan panelis terhadap minuman emulsi VCO. Konsentrasi jahe merah dan sereh divariasikan: 100% jahe merah, 100% sereh, 50% jahe merah : 50% sereh, 75% jahe merah : 25% sereh, dan 25% jahe merah : 75% sereh. Hasil menunjukkan bahwa konsentrasi jahe merah dan sereh mempengaruhi warna minuman emulsi VCO yang dihasilkan. Penggunaan gum arab (7%) menghasilkan minuman emulsi VCO yang stabil selama waktu pengamatan 7 hari. Total gula (% sukrosa) berkisar pada 26,00%-28,67%. Total fenol dan bilangan peroksida minuman emulsi berkisar dari 20,92 – 28,61 mg GAE/ g sampel dan 0,08-0,79 mg ekuivalen O2/kg. Pada konsentrasi 15 ppm, aktivitas antioksidan minuman emulsi berkisar antara 58,58% - 71,20%. Minuman emulsi dengan penambahan 100% jahe merah paling disukai dalam aroma, warna, dan penampakannya, sedangkan minuman emulsi dengan penambahan 75% jahe merah dan 25% sereh yang paling disukai rasanyaVirgin Coconut Oil (VCO) as one of the coconut products with many health benefits hard to be consumed directly because of its oily taste. Therefore, to make it more tastier to consume, VCO will be made in form of emulsion drink. In this research, VCO emulsion drink will be added with red ginger and lemongrass to investigate the addition effect of these spices on the physical, chemical characteristic, antioxidant activity, and panelist preference rate of its emulsion drink. The concentrations of red ginger and lemongrass were varied : 100% red ginger, 100% lemongrass, 50% red ginger : 50% lemongrass, 75% red ginger : 25% lemongrass, and 25% red ginger : 75% lemongrass. The result shows that the concentration of red ginger and lemongrass affected the color of the emulsion drink. The addition of arabic gum (7%) produced a stable emulsion drink during the observation time of 7 days. The sucrose percentage of the emulsion drink is 26,00% to 28,67%. The average of total phenol and peroxide numbers in the emulsion drinks was 20,92 to 28,61 mg GAE /g sample and 0,08-0,79 mg equivalent O2 / kg. At a concentration of 15 ppm, the antioxidant activity of emulsion drinks ranged from 58,58% - 71,20%. VCO emulsion drinks with the addition of 100% red ginger were the most preferred in terms of aroma, color, and its appearance, while the addition of 75% red ginger and 25% lemongrass were the most preferred in taste
Aedes aegypti is the most dominant vector in the transmission of dengue hemorrhagic fever (DHF). In addition to Ae. aegypti, Ae. albopictus is a secondary vector of the dengue virus, and both species are widespread in Indonesia. The dengue virus is transmitted from person to person through the bite of an Aedes spp. The vertical (transovarial) transmission of the dengue virus from infective female mosquitoes to their offspring is one of the means by which the dengue virus maintains its existence in nature. Transovarial dengue virus transmission in Aedes spp. mosquitoes contributes to the spread and maintenance of the dengue epidemic. This study employed a qualitative survey to detect dengue virus transovarial transmission in Ternate using the streptavidin-biotin-peroxidase complex (ISBPC) immunohistochemical test. The ISBPC examination of samples collected from the four subdistricts in Ternate revealed a positive result for transovarial transmission of dengue virus. Four Aedes spp., including two Ae. aegypti females, one Ae. albopictus female, and one Ae. albopictus male, tested positive for transovarial transmission of dengue virus in the district of North Ternate. Four Aedes spp., including three Ae. aegypti females and one Ae. aegypti male, were found to be positive for the transovarial transmission of dengue virus in the Central Ternate district. Seven Aedes spp., including five Ae. aegypti females, one Ae. aegypti male, and one Ae. albopictus female, tested positive for transovarial transmission of the dengue virus in the district of South Ternate city. One Ae. aegypti male showed positive results for transovarial transmission of dengue virus in the Ternate Island District. In this study, the transovarial transmission of the dengue virus occurred in both Aedes spp. female and male mosquitoes. It was demonstrated that Aedes spp. carry the dengue virus in their ovaries and can pass it on to their offspring. As a result, the cycle of passing the dengue virus on to local mosquito populations in the city of Ternate is not going to end just yet.
This study aims at determining changes in the level of carbohydrates and protein during the storage process. The results show that during six-month storage, there are decreases in the levels of carbohydrates and protein content in rice grains. Decreased levels of carbohydrate occurred on red rice obtained from Manado Bersehati market (52.23%), followed by red rice from Karombosan market (41.21%). Control rice with insects and fungi protection treatments' experienced the smallest level of carbohydrate decrease (9.20%). The largest protein content decreased occurred in red rice obtained from Bersehati Market (5,45%), followed by red rice from Karombasan market (4.93%). The smallest decrease occurred in the control rice Superwin obtained from Market Bersehati (0.96%). Long period storage affects the quality of rice.
Basil (Ocimum basillicum L.) is well-known as a medicinal plant and culinary herb because of its phytochemical contents. Crude extracts of basil leaves are of particular interest for health benefits, including in reducing blood glucose level. Nanochitosan is potentially applied to increase the efficacy of phytopharmaceuticals due to its low viscosity that easily absorbed by the tissues. This study was conducted to evaluate the antihyperglicemic capacity of basil leaf extracts when coated with nanochitosan. Nanochitosan was prepared from the parrot fish (Scarus sp) scales derived Chitosan by using the gelatin ionic method. Both chitosan and nanochitosan were characterized by Fourier Transform Infrared Spectroscopy (FTIR), SEM, particle sizing and heavy metals analyses. Extraction of basil leaves were performed by a maceration method using ethanol 96% and the extract was evaporated. Nanochitosan was then applied as coatings of the extracts, and the nanochitosan coated extract was separated from the solution by centrifugation. In vivo assay was applied using 24 male rats, which divided into extract treated groups and the control groups. All the animals were fasted for 12 h, but were allowed free access to water, before commencement of the experiments. Hyperglycemia condition was induced by a single intraperitoneal dose of 120 mg/kg. The rats were fasted for 12 h and blood was taken from the tail artery of the rats. The extract of basil leaves at the dose of 400, 800, 1000 mg/kgBW significantly lowered blood glucose level (P<0,05) of rat in hyperglycemic after 5 hours of extract administration. The efficacy of antihypeglicemic dose of basil leaves extracts coated with nanochitosan was found to be 400 mg/kgBW. Based on the result of the study, it is suggested that nanochitosan could increase the efficacy of phytopharmaceuticals.
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