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Aims: Correlations between DNA content of trichothecene-producing Fusarium spp. and concentration of the key mycotoxin deoxynivalenol (DON) in cereal samples. Methods and Results: A LightCyclerÔ PCR-based assay was used to quantify the DNA from trichothecene-producing Fusarium spp. in 300 wheat samples. DNA concentrations ranged from not detectable to 16AE3 mg kg )1 whereas DON concentrations (GC/MS data) varied from not detectable to 34AE3 mg kg )1 . Data analysis revealed a coefficient of correlation r ¼ 0AE9557 between DON concentrations and DNA-amounts over all samples. An interval of confidence for P ¼ 95% was calculated based on samples with DON concentrations £ 1AE5 mg kg )1 (n ¼ 234). Conclusions: Quantification of 32 samples of Fusarium-contaminated wheat was performed within 45 min. Data analysis allowed estimation of DON contamination from quantitative PCR data in the wheat samples. Significance and Impact of the Study: The method described is useful for the screening of cereals in industrial quality control.