At first two-layer HA-ZnO/Alg coating was obtained on the preanodized Ti6Al4V substrates by the thermal substrate deposition method (TSD). In frame of this work TSD method was at first applied for obtaining ZnO coating from aqueous alginate-containing solutions on a pre-deposited OH layer. XRD, SEM analyzes show that the biopolymer has a significant influence on the coatings formation, their morphology, texture, structure of ZnO nanoparticles. The average rate of HA deposition and ZnO deposition from alginate containing solution are 4 μm/min and 30 μm/min, respectively. In the presence of alginate boll-shaped ZnO microparticles are formed on the HA layer, consisting of needle-shaped crystals. Obtained coating is promising for medical implants, based on titanium and its alloys, because they potentially have both bioactivity and antibacterial activity.