This paper reports the fabrication and testing of two configurations of optical sensor systems based on Surface Plasmon Resonance (SPR) at the interface of a liquid sample and sandwiched structures realized starting from the exposed core of a Plastic Optical Fiber (POF). The proposed geometries have proven to be suitable for measuring the refractive indexes of liquids whose refractive index falls around 1.35. Furthermore, the proposed sensing head, being low cost and relatively easy to realize, may be very attractive for biosensor implementation.

We present a technique for dynamic strain measurements in optical fibers based on the stimulated Brillouin scattering interaction between two counterpropagating optical pulses. The technique allows for a high sampling rate and permits to addressing dynamically and randomly the position at which vibration is measured. Preliminary experimental results carried out with a perturbation frequency up to 98 Hz demonstrate the validity of the proposed technique.

Chordin is a bone morphogenetic protein (BMP) inhibitor that has been identified as a factor dorsalizing the Xenopus embryo. A novel secreted protein, CHL (for chordin-like), with significant homology to chordin, was isolated from mouse bone marrow stromal cells. Injection of CHL RNA into Xenopus embryos induced a secondary axis. Recombinant CHL protein inhibited the BMP4-dependent differentiation of embryonic stem cells in vitro and interacted directly with BMPs, similar to chordin. However, CHL also weakly bound to TGFbetas. In situ hybridization revealed that the mouse CHL gene, located on the X chromosome, was expressed predominantly in mesenchyme-derived cell types: (1) the dermatome and limb bud mesenchyme and, later, the subdermal mesenchyme and the chondrocytes of the developing skeleton during embryogenesis and (2) a layer of fibroblasts/connective tissue cells in the gastrointestinal tract, the thick straight segments of kidney tubules, and the marrow stromal cells in adults. An exception was expression in the neural cells of the olfactory bulb and cerebellum. Interestingly, the spatiotemporal expression patterns of CHL were distinct from those of chordin in many areas examined. Thus, CHL may serve as an important BMP regulator for differentiating mesenchymal cells, especially during skeletogenesis, and for developing specific neurons.

The experimental validation of a numerical technique for temperature/strain profile reconstruction based on Brillouin optical-fibre time-domain analysis (BOTDA) sensors is presented. In this approach, we search directly for the Brillouin frequency shift profile along the fibre that matches the measured data. The algorithm is based on a harmonic expansion of the unknown profile, whose coefficients are determined by means of a multidimensional minimization. Experimental measurements have been carried out in order to reveal the influence of nonlocalities in Brillouin measurements, and to prove the capability of the proposed algorithm to compensate for these effects.