No abstract

No abstract

The species Myrciaria floridunda O. Berg, popularly known as cambuí, belongs to the Myrtaceae family. Cambui is a native, non-endemic species that occur in diverse environments in Central America and South America. They are slow-growing plants with a shrub or sub-shrub habit. The fruits, the product of interest of the species, are small, spherical berries orange or red in colorturning to wine colorwhen they are ripe. The exploitation of the species is still mostly extractivist, carried out by traditional local families who, in times of fruiting of the species, leverage their income by selling fruits at fairs. The fruits can be eaten fresh, in the form of jellies, liquor or wine. To study the genetic diversity of the species using ISSR-type molecular markers, it is necessary to first isolate DNA in sufficient quality and quantity. Here, leaves for DNA extraction were collected from the active germplasm bank of the Federal University of Alagoas, Brazil. The DNA of the species was extracted using CTAB detergent methodology with modifications adapted to the species. Twelve ISSR primers were tested on DNA from two cambui genotypes. Of the twelve primers, eight were selected due to their polymorphism index above 50%, namely: UFAL-2, UFAL-3, UFAL-5, UFAL-6, UFAL-7, UFAL-8, UFAL-9 and UFAL-10.

No abstract

In order to assure and evaluate the genetic diversity, wild populations of Cotton (Gossypium mustelinum) were collected and evaluated from the coastal plain north of Pernambuco, Brazil. Such populations occur in urban areas in a state of real expansion and with imminent risks of extinction. As a result of these risks and the state of real expansion, aiming at the ex situ conservation of these genetic resources, branches of 66 plants were collected in three populations of G. mustelinum that are located in restinga vegetation in the localities of Ponta de Pedras and Bara of Catuama, both in the municipality of Goiana and in the locality Sossego Beach in the municipality Island of Itamaracá. The collected genotypes were inserted in a new Germplasm Bank (BAG) at the Federal Rural University of Pernambuco, after which a sample composed of 24 genotypes contained in the BAG was collected to perform genetic diversity studies using molecular markers of ISSR type. For the molecular analysis, 24 accesses with 4 ISSR primers were analyzed, which produced a total of 36 bands, with a mean of 1,52 alleles per amplified locus. The genetic dissimilarity values, calculated according to the complement of the Jaccard index, ranged from 0.000 to 0.080. The UPGMA method grouped the accesses into three groups. The UFRPE30, UFRPE42 and UFRPE45 accessions were more dissimilar and UFRPE-48, UFRPE-50, UFRPE-52, UFRPE-55, UFRPE60, UFRPE06, UFRPE28, UFRPE29, UFRPE1, UFRPE2, UFRPE17 the least dissimilar. The ISSR markers used in this study demonstrated efficiency in the detection of molecular polymorphisms, revealing genetic variability among the 24 accessions. Considering the results obtained in this work, it is possible to infer that there is considerable genetic variability among the accessions of cotton, demonstrating the importance of the markers in the analysis of variability of species not studied, such as (G. mustelinum).