The current study sought to investigate the anti-fatigue efficacy of poly herbal granule (PHG) formulations of the extracts of Asparagus racemosus, Chlorophytum borivilianum, Tinospora cordifolia, Tribulus terrestris and Withania somnifera enriched with general tonic and health promoting properties based on Aloe vera gel and soyabean whey in Swiss mice. The nutrient compositions of the selected PHG exhibited that the carbohydrates were the major constituents in both AVII and SBWII, followed by protein and total ash. Fat contents were 0.96 and 1.18 g100g -1 in AVII and SBWII, respectively. The energy levels of AVII and SBWII were 378 and 377 kcal100g -1 , correspondingly. Higher contents of Ca (70.20 mg100g -1 ), Mg (85.27mg100g -1 ) and P (61.55 mg100g -1 ) were observed in SBWII. The trace elements revealed that, Zn and Fe content were 1.84 and 8.63mg100g-1 in AVII and 1.85 and 7.85mg100g -1 in SBWII, respectively. The retention and stability of Vitamin C, total sugar, reducing sugar and non-reducing sugar for 270 days illustrated the storage life of the product in ambient condition. The body weight of mice, swimming time and corresponding biochemical parameters including haemoglobin, serum protein and blood glucose level were measured by standard method. The oral administration with PHG supplement for 30 days markedly increased the body weight (11.40-11.80%), haemoglobin (11.82-12.46%), serum protein (3.77-6.02%) of the mice. Following forced swimming test (FST), prolonged exhaustive exercise time of 231.02 (AVII), 237.50 (SBWII) minutes were recorded for PHG supplemented groups which were 36.23 (AVII) and 37.97 (SBWII) per cent longer than that of the control group. The minute increase of blood glucose levels (4.51-4.37%) in PHG supplemented Swiss mice after FST as compared to tremendous increase in blood glucose level (40.07%) in the control group illustrated the anti-fatigue activity of PHG. K e y w o r d s