SUMMARYThis research was carried out on 6 barley varieties (two-rowed; Otis, Nure, Pewter Fouga, Prestige and six rowed; Athenais) in a Randomized Complete Block Design with three replications in 2008-2009 growing season in Cyprus; Turkmenkoy, Kumkoy, Tepebas and Haspolat locations. It was determined that the effects of genotype, environment and genotype x environment interaction were significant at 1% level of probability for grain yield. Grain yield varied from 61.0 -410.5 kg/da. Kumkoy location had the highest grain yield (303.7 kg/da) while Turkmenkoy location had the lowest grain yield (118.3 kg/da). While, cultivars Nure and Pewter had the highest yield (250.0 and 261.4 kg/da), cultivars Fouga and Prestige had the lowest grain yield (156.3 and 158.0 kg/da) for all environments. While the highest grain yield is found in Kumkoy location with Nure (410 kg/da) and Pewter (400 kg/da), Turkmenkoy location has the lowest grain yield with Fouga (61 kg/da) variety.