Schiff base compounds are reported to have various biological activities, one of which is antibacterial. This study aims to determine the characterization of the Schiff base product with the sonication method and to determine its antibacterial activity. Schiff base products from 4formylpyridine and panisidine were characterized using FTIR, KG-SM, and 1H-NMR as well as antibacterial activity tests. The results of the characterization using FTIR produced a wave number of 1621 cm −1 which was thought to be the absorption of the C = N group which was the target compound, and the KG-SM characterization showed the presence of the target compound for the synthesis of 4-methoxy-N(pyridine-4-ylmethylene)aniline at m/ z 212. Meanwhile, the 1H-NMR characterization of the product showed the presence of a singlet proton imine signal at a chemical shift of 8.46 ppm (1H, s), as well as the presence of antibacterial activity that was effective in inhibiting bacterial growth at a concentration of 10,000 μg/mL.