Common bean (Phaseolus vulgaris L.) is the main source of protein, fiber, and iron for Guatemala's poorest households. Bean rust (caused by Uromyces appendiculatus (Pers) Unger) can reduce seed yield up to 100%. There is limited information about U. appendiculatus virulence diversity in Guatemala, especially in the mid-altitude highlands. To address this limitation, in 2015, 23 U. appendiculatus samples were collected in the Western Guatemalan Highlands and characterized as races 63-1 and 31-1 using the rust differential set. These two races, along with the newer race 20-3 found in North Dakota, were used to evaluate the reaction in 372 climbing bean accessions from Guatemala. Most of the accessions were resistant: 82% to race 63-1, 86% to race 31-1, and 90% to race 20-3. Based on a genome-wide association study (GWAS) using 78,754 single nucleotide polymorphisms (SNPs), regions on Pv02