The present study was conducted to evaluate the effect of Cumin on diabetes mellitus induced by alloxan in rats. Male Wister rats were used, and randomly divided into five groups (6-8 rats for each group): The first group: was administered distilled water and served as normal control, the second group: induced diabetes by single subcutaneous injection of alloxan 100 mg/kg.body weight and served as diabetic control, the 3,4,5 groups was administered 50,100,200 gm/kg of rat's forage respectively for four weeks, then diabetes are induced in this groups by same route as above. Cumin treatment in all doses lead to significant decrease glucose levels, and triglyceride and significant decrease in cholesterol level in dose 100, 200 gm/kg of forage and no change occur in total protein level. These results indicate that cumin have a role in delaying of diabetes through the effects of cumin in decrease some biochemical parameters.
The study was conducted at the Ruminant research Station of the Livestock Research Department-Agricultural Research department-Ministry of Agriculture. Using 24 Yearlings Turkish Awassi, divided into four groups T1, T2, T3 and T4 equal number by 6 Yearlings, Three Turkish Awassi rams were selected for natural breeding, Each ram was implant with three melatonin implants Subcutaneous the ear in a day 28 th October-2017, After 8 days of implant rams, Yearlings of the T1 and T2 groups were implant with one melatonin implant Subcutaneous the ear, After 1 month of female implant melatonin, the T2 and T3 groups were treated with vaginal progesterone sponge for 14 days. The groups were then injected with eCG 300 IU and T4 was the control group. Blood samples were taken jugular to estimate the serum progesterone level for days 0, 14 and 28 after the removal of sponges. The rams were release on 0 of removing the sponges to mate all the yearlings of the study and left with it for 51 days. The results showed that there were significant differences in the studied characteristics of the treatments, where the highest T2 and T3 at time, the number and percentage of estrus and the period from the enter of rams to birth exceeded the rest of the treatments, While T2 was the highest in female births, fertility rate and number of births. No infection or adhesion was observed in treatment T2 other than T3. Treatment with melatonin improved the level of progesterone. No significant difference was observed in the weight of females, sex and weight of births between the experimental factors. In conclusion, treatment with melatonin only or with progesterone was a good way to improve the reproductive and productive performance of sheep.
The study was conducted on know the effect of different levels of local Bentonite in a percent of 0, 1 and 3% to the ration of Awassi lambs on concentration of minerals and Ammonia concentration and the numbers of microflora of ruminal fluid. Fifteen Awassi lambs were used in this study, aged between 4-6 months with mean body weight of 27.57 ± 0.71 kg. The animals were an individual pens in a space of 1.5 × 2 m. The animals were divided randomly into three equal groups (5 lambs/ groups). The 1st group regarded as a control group, While the 2nd and 3rd group adding to its concentrated ration the local Bentonite in a percentage of 1 and 3% respectively. The animals were fed on a concentrated ration 3% of the body weight and the rough ration (alfalfa hay) were given ad libtium. The results of experiment showed there were a significant increase (P<0.05) in concentration of Ammonia nitrogen of ruminal fluid in the control and 3rd group (3% Bentonite), before morning fed. While there were significantly (P>0.05) decrease in ruminal fluids of animals fed at the 2nd and 3rd group ration (1, 3% Bentonite) after two hours of morning fed as compared with the control group. The results also showed after four hours of morning feeding there was no significant difference between different groups in concentration of Ammonia in ruminal fluid. There was a significant decrease (P>0.05) in sodium salts in blood of lambs fed on 2nd and 3rd ration as compared with control ration. The results showed that addition of Bentonite at different levels (0, 1 and 3) % on lamb ration have no effect on concentration of blood Ca, Ma, K and Al. It has been observed that there was a significant increase (P<0.01) in the numbers of ruminal fluid microflora after addition of 3% Bentonite as compared with control and 1% Bentonite. It was concluded from this study that addition of Bentonite to the lamb ration have a beneficial effect on blood sodium, ruminal fluid Ammonia and the numbers of ruminal microflora.
The study was conducted to know the level of Malondialdehyde (MDA) in seminal plasma of cryopreserved semen of Holstein bulls after addition of zinc sulphate, cysteine, PGF2α and their combination in vitro. Semen was collected from 7 Holstein bulls, presented in Artificial insemination Center which belonged to the Directorate of Animal Resources/ Ministry of Agriculture at Abu-Graib at the west of Baghdad. Pooled semen were diluted with Tris- based extender and divided into five parts. The first part (T1) serve as a control (without addition). The 2nd part (T2) added to it zinc sulphate (0,576 mmol/ ml). The 3rd part (T3) added to it cysteine (5 mmol/ ml). The 4th part (T4) added to it PGF2α (37.5 pg/ ml). while the 5th part added to it a combination of previous substances at the same concentration. They packed in straws and cryopreserved in a liquid nitrogen and after 30, 60 and 90 days. Seminal plasma when isolated to measure the level of MDA. The results showed a significant decrease (P>0.01) in MDA level in the combination treated group (zinc, cysteine and PGF2α) 0.450 ± 0.11 (mmol/ ml) as compared with control group 1.025 ± 0.38 (mmol/ ml), zinc 0.867 ± 0.12 (mmol/ ml), cysteine 1.06 ± 0.12 (mmol/ ml) and PGF2α group 0.968 ± 0.17 (mmol/ ml) respectively. It was concluded from this study that addition of a combination of zinc, cysteine and PGF2α to the Holstein bull semen could decrease the level of MDA which might be due to the synergistic effect of these substances.
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