A new method has been developed by preparing complexes involving condensation of amino acids with 8-hydroxyquinoline and 8-hydroxyquinaldine. The products so obtained are being investigated for identification and quantitative estimation of amino acids using different spectroscopic techniques including fluorescence activity of newly synthesized products. The method adopted in our laboratories is rapid, versatile, reproducible and provides excellent results for adoption by analytical, agricultural and biomedical laboratories to estimate amino acids and metals in composite matrix at economical costs. 8-hydroxyquinoline (Oxine) and 2-methyl-8-hydroxyquinoline (8-hydroxyquinaldine) condensed with valine produced fluorescent complexes. The complexes have been investigated for identification and quantitative estimation of amino acids. By this method identification of amino acids in nano mole quantities has become possible by fluorescence activity of valine-8-hydroxyquinoline and valine-8-hydroxyquinaldine complexes involving different excitation and emission wave lengths. This fluorescence activity of complexes is 100 to 1000 times higher than assay method involving ninhydrin and amino acid analyzer.

A new method has been developed by preparing complexes involving condensation of amino acids with 8-hydroxyquinaldine using various experimental conditions. The products so obtained are being investigated for identification and quantitative analysis using different spectroscopic techniques including fluorescence activity of newly synthesized products. In present studies the identification of amino acids in nano mole quantities has become possible by fluorometric activity of amino acids-quinaldine complex involving different excitation and emission wavelengths. This fluorometric activity of complexes is 10 to 100 times more sensitive method than assay method involving ninhydrin. 2-methy1-8-hydroxyquinoline (8-hydroxyquinaldine) condensed with Alanine and Phenylalanine produced fluorescent complexes. The complexes separately have been investigated for identification and quantitative estimation of amino acids. The use of 8-hydroxyquinaldine for various purposes and its comparison to 8-hydroxyquinoline for similar purpose indicates decrease in fluorescence signal of 8-hydroxyquinaldine-amino acid complexes.