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The present study describes the clinical signs, diagnosis via microscopic faecal examination and treatment of Buxtonella sulcata infection in 26 Surti buffalo calves. The study was aimed to control diarrhea caused by Buxtonella sulcata. Buxtonella sulcata infection or buxtonellosis is an important pathogenic factor for causing diarrhoea in suckling and post-weaning calves. Buxtonella sulcata infection is found more frequently associated with other parasitic infections that cause diarrhea. It is also described by inanition, rough body coat and lacrimation apart from generalized weakness and diarrhoea. All the calves were successfully treated with parenteral medicinal therapy using antiprotozoal, antiinflammatory and anti-histaminics for five days.