A novel selenium nanobiocomposite consisting of X-ray amorphous nanoparticles of red elemental selenium (average size ~67 nm) uniformly encapsulated into arabinogalactan macromolecules has been synthesized. The effect of this nanobiocomposite and its precursors (pure arabinogalactan and selenium dioxide) on the viability of a phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (Spieck et Kotth.) Skapt et Burkh (C. michiganensis) and potato plants has been investigated in vitro. The bactericidal effects of free selenium dioxide and the nanocomposite obtained from the former (normalized selenium concentration 0.000625%) have been demonstrated. The targeted metabolic (trophic) delivery of antimicrobial selenium nanoparticles to the bacterial cells by the arabinogalactan matrix incorporating the particles was detected in the latter case. The treatment of potato plants with selenium dioxide was found to have a negative effect on peroxidase activity and plant growth. On the contrary, treating the plants with the selenium nanocomposite had no negative effects. Thus, the nanocomposite of elemental selenium and arabinogalactan produced in the present work can be considered a new antimicrobial agent for plant sanitation with minimal side effects.

1 Федеральное государственное бюджетное учреждение науки Сибирский институт физиологии и биохимии растений Сибирского отделения Российской академии наук, Иркутск, Россия, irina170885@mail.ru 2 Федеральное государственное бюджетное образовательное учреждение высшего образования «Иркутский национальный исследовательский технический университет», Иркутск, Россия Аннотация. Применение нативного электрофореза при разделении растительных белков в хвое Pinus sylvestris позволило получить и сравнить данные о влиянии изменений факторов окружающей среды на изоферментный состав пероксидазы в растении. Установлен ряд зависимостей изоферментного состава пероксидазы хвои Pinus sylvestris от условий окружающей среды, используя средства языка программирования R.Abstract. Use native electrophoresis in separating plant proteins in the needles of Pinus sylvestris has allowed to obtain and compare data on the impact of environmental factors changes Wednesday on izoenzyme composition of peroxidase in the plant. A number of izoenzyme peroxidase composition dependency of needles of Pinus sylvestris from ambient conditions of environmental, using the R programming language.

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