On a field level among poultry flocks a study was conducted to estimate the efficacy of the most common disinfectants against E. coli (APEC isolates) was determined. The selected disinfectants were; Formalin, Phenol, QAC, Halamid, Virkon" S and Micro Sept M against E. coli isolates of commercial egg- layer flocks in Egyptian farms. The recovered results showed that: (1) the incidence of E. coli in the observed commercial egg-layer flocks; the isolation of E. coli (APEC of rfbO157 gene ) from 4 commercial egg-layer flocks (26.7 %) out of 15 flocks, the mortalities rates at the end of 78 weeks of age was 20% and also, the current egg-production, average egg weight, hen housed day, hen housed egg and percent peak of egg-production were: 69.2%,58.3 gm, 70%,326,78% and 69%,respectivel. (2) Formalin, Micro Sept M and Virkon'S treatment demonstrated a significant reduction in E. coli populations especially by cold fogging method.
The recovered results showed that: the incidence of Salmonella typhymurium in the observed commercial egg-layer flocks; the isolation of Salmonella typhymurium from 5 flocks (33.7 %) out of 15 flocks, the mortalities rates at the end of 78 weeks of age was 16 % and also, the current egg-production, average egg weight, hen housed day, hen housed egg and percent peak of egg-production were: 68 %,59.2 gm, 68.4%, 318, 78% and 70%, respectively. The efficacy of the most common disinfectants against Salmonella typhymurium was determined. The selected disinfectants were; Formalin, Phenol, QAC, Halamid, Virkon'S and Micro Sept M against Salmonella typhymurium isolates of the studied commercial egg- layers in Egypt (hfq gene of Salmonella typhimurium). It was observed that, S. typhimurium affected significantly only by Micro Sept M and Virkon'S, at low rate of application; the Log 10 of populations after15 mint exposure were 4.2, 4.4, respectively the recovered results showed also, that: Formalin, Phenol, Micro Sept M and Virkon'S treatment demonstrated a significant reduction in Salmonella typhymurium populations at high rate of application. It is evident from the results using formalin, phenols and QAC at concentration of 4% ,5% and 33.3 % by fogging other than spraying had increased action on the tested pathogens S. typhimurium after 15 min contact time (35.7%,42.8 % and 76.8%, respectively).
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