An attempt was made to evaluate anti-anaemic properties of probiotic Moringa oleifera leaf extract using animal model. Twenty four male rats were randomly assigned to four groups and all the animals were given Phenylhydrazine orally to induce anaemia. Enterococcus durans was selected as probiotic supplement. Group 1 was provided with 300mg/kg of M.oleifera leaf extract. Group 2 was supplemented with 0.3 ml of E.durans along with 300mg/kg of M. oleifera leaf extract. Group 3 and 4 were fed with E.durans and iron supplement (1ml/rat) respectively. Blood samples were collected on a weekly basis and various haematological and biochemical parameters were analysed. The haemoglobin level was found to be higher with 14.9±0.25 g/dl for rats fed with probioticated M.oleifera leaf extract. E.durans has proliferated in gut which was verified through faecal and gut microbiota analysis. Hence, probiotic M.oleifera leaf extract was found an effectual nutraceutical supplement to treat anaemia.