This study was performed to evaluate the protective effects of feeding reduced glutathione (GSH) against hepatic dysfunctions related to dietary aflatoxicosis in Inshas chickens (a local Egyptian chicken strain). 5 ppm of GSH were used with 500 ppm of aflatoxin B1 (AFB1) /kg diet administrated. In total, 96 chickens were divided into 4 treatments with 3 replicates. T1 was the negative control. T2was the positive control group (AFB1). Groups T3 and T4 were administrated with GSH, and GSH+ AFB1, respectively. At the end of the experiment (28-38) wk of age, samples were collected for analysis. The results showed that AFB1 has significant potency for increasing aspartate transaminase (AST), creatinine in blood, thiobarbituric acid reactive substances (TBARS) in muscle , while reducing the plasma α-tocopherol content, antioxidants enzymes (glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD) and total antioxidant capacity) in blood of chicken. In addition, the negative effects of AFB1 on mRNA for antioxidant enzymes (GPX, SOD and CAT), hepatosomatic index (HSI), lipid peroxidation on birds aflatoxicosis were recorded in this study, also the efficacy of GSH for ameliorating aflatoxicosis were recorded. Moreover, these results showed that the ability of GSH when use with aflatoxicosis to counteract the toxic effects of AFB1 on chickens. Additionally, the GSH-Px (T3) were enhanced the antioxidative status. Finally, the optimal levels of GSH and method of used for detoxification of aflatoxin effects need more studies.