Obesity is excess of weight due to accumulation of fat which can cause dyslipidemia. One of dyslipidemia sign is increasing of total cholesterol level. The saponin, tannin and flavonoid as antioxidants components within kepok banana peel are predicted could decrease total cholesterol level. This study aims to determine whether saponin, tannin and flavonoid in kepok banana peels are effective against total cholesterol level in obese mice. This study was a true experimental using 20 obese male mice (Mus musculus L.) strain Deutschland-Denken-Yoken (ddY) and divided in four groups, which are normal control group (K1), obese control group (K2), and groups that were given extract of kepok banana peel treatment with dose 8,4 mg/day (KP1) and 16,8 mg/day (KP2). The treatment were given in 14 days. Total cholesterol level of each group was measured by spectrophotometer. The results obtained p=0,000, in one-way ANOVA test. Furthermore, in the Post Hoc Test generally found that there was significant differences between groups. There is effect of giving kepok banana peel to decreased total cholesterol level of obese mice. The effect of kapok Banana peel extract level of 8.4 mg/day more effectively lower total cholesterol level compared to banana peel extract level of 16.8 mg / day. The anti-cholesterol effect of banana fiber ethanol extract proved to decrease total cholesterol in obesity male mice (Mus musculus L.) strain ddY.