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I Inactive or nonfunctional ovaries are one of the most important reported causes of the prolonged postpartum anestrum that resulted to the delayed onset of estrus and inefficient breeding in buffaloes. The suggested study of progesterone in the blood serum of buffalo could be potentially useful as far as the hormone content that might reflect the postpartum condition in the buffalo-cows. This study aimed to evaluate the effect of exogenous progesterone in treatment of ovarian inactivity in the Egyptian dairy parturient buffalo-cows. This study was carried out on parturient buffalo-cows came to veterinary clinic and owned by small holders. These buffalo-cows ages ranged between 2-5 years. The animals were divided into 4 groups (28 animals). First group (7 buffalo-cows) as control group without any treatment. The second group (5 buffalo-cows) to study the effect of Progesterone (prontogest®) + PGf2α treatment trial. Third group (7 buffalo-cows) to study the effect of CIDR treatment trail. Forth group (9 buffalo-cows) to study the effect of CIDR+GPG protocol as a treatment trail. Progesterone level was measured before treatment (for diagnosis) and after treatment (to detect the response of treatment). The results indicated that using CIDR+GPG gave good results for the treatment of ovarian inactivity in buffalo-cows, where increased the incidence of estrus and improved the reproductive parameters in dairy parturient buffalo-cows.

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This study aims to study the follicular changes during the estrous phase of 2 years pubertal mares showed previously signs of estrous and corpora lutae were detected on their ovaries. Five fillies of 2 years old were subjected to daily ultrasonographic examination for 18 days. Follicular dynamics were studied during estrus from day 6 before ovulation (-6) till day of ovulation (day 0). Follicles were classified into small (<10-15mm), medium (>15-<20) and large (>20). Number and diameter of each follicle was measured. Data was subjected to simple one way ANOVA to study the effect of day on number and growth rate of each follicle class. Results revealed that no significant variation was observed in number of all follicle classes. The dominant follicle after deviation to dominance starts to grow from >26mm in diameter to reach a maximum diameter of 42.3 mm on day of ovulation. At least one large follicle is dominant and the other large follicle is subordinate three days before ovulation. Compared to the day 6 before ovulation, the day before ovulation has significantly low large follicle number. There was a decrease of number and diameter of medium follicles on day 2 before ovulation. This study indicated that after ovulation fillies became much similar to mature and older mares. High genetic value fillies could be subjected to oocyte collection programs before enrolling her to either breeding program or performance.