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In embryonic and neoplastic cells suspended in culture medium, incorporation of labelled glucosamine into glycoprotein occurred in three distinct subcellular sites: endoplasmic membranes, ribosomes, and cell sap, of which the first was quantitatively the most important. The presence of labelled glucosamine on polysomes indicates that the carbohydrate moiety starts to become attached to the polypeptide while the glycoprotein is still linked to the polysome.

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