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A radiochemical method for the determination of 210 Pb in various types of samples was validated. An extraction chromatographic material Eichrom SrResin ® was used for Pb separation. From the resin, Pb was eluted with 6 M HCl. The method was tested for water, inorganic and organic matrices. As a main result of the study, activity concentrations of 210 Pb in 19 food samples of the main Czech food-basket items were determined. Results ranged from 0.03 ± 0.01 Bq kg -1 to 1.54 ± 0.40 Bq kg -1 , fresh mass.