We induced somatic embryogenesis from the cotyledon segments of O/ea europaea (L) cvs. 'Chetoui', 'Chemleli', and 'Arbequina'. Calli were established from all three cultivars on OMc media supplemented with IBA and 2i-P. The greatest success was obtained with media that contained zero or low concentrations of growth regulators. High levels of hormones (i.e.,>0.5 mgL -1 IBA and 2i-P) inhibited embryogenesis. Embryos at different maturation stages were observed with continuously proliferating secondary embryogenesis. Abnormally shaped embryos and teratoma were also noted. Four weeks was the optimal incubation period for inducing embryogenesis on the auxin-containing medium. In addition, 30 to 40 gL -1 sucrose was more effective than glucose in stimulating the growth and maturation of somatic embryos. Embryogenic efficiency was also higher when multivariate combinations of nitrogen sources (inorganic and organic nitrogen forms) were used. The plantlets that were derived from our germinating somatic embryos were similar to those obtained from axillary buds.

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