Soy proteins are used in products of pork butchery for their binding properties and/or their protein content. French legislation limits the addition of binding proteins to 2% in some meat products. Current detection methods are unable to quantify vegetable proteins in these products: the results differ according to the manufacturing process. We have developed a method to quantify these proteins by using immunohistochemical techniques associated with image analysis. This process is based on measuring the areas occupied by labeled soy proteins in sections mounted on slides, and not on estimating amounts of proteins in solution.

A set of measurements have been conducted in order to determine the annual effective dose resulting from the ingestion of natural radionuclides present in eight different brands of bottled water commercialised in Tunisia. Using high-resolution gamma spectrometry technique, we have noted the presence of the following radionuclides: 214Bi, 226Ra, 228Ac, 212Pb, 235U and 40K. For all classes of age, only the radium concentration was found to be significant in the calculation of the equivalent dose. Some of the studied samples exceeded the reference level of 0.1 mSv y(-1) of effective dose.

Résumé -Introduction : le kératokyste odontogéne (KKO) est une des tumeurs odontogènes les plus fréquentes. Plusieurs travaux se sont intéressés à l'étude de cette lésion depuis sa première description afin de pouvoir comprendre son comportement biologique. Matériel et méthode : il s'agit d'une étude rétrospective immunohistochimique des marqueurs PCNA, Ki67 et P53 chez 40 patients opérés pour un KKO. Résultats : ces marqueurs sont retrouvés dans les couches suprabasales de l'épithélium de la paroi kystique. Il n'y a pas de différence statistiquement significative dans l'expression de ces marqueurs entre les différentes formes cliniques (sporadique, récurrent et syndromique), et il n'existe pas de corrélation entre ces marqueurs et la survenue d'une récidive. Conclusion : PCNA, Ki67 et P53 ne sont pas des marqueurs pour l'histopronostic des KKO.Abstract -Immunohistochemical study of proliferative (Ki67, PCNA) and apoptotic (P53) factors of 40 cases of odontogenic keratocysts. Introduction: the odontogenic keratocyst (OKC) is one of the most prevalent odontogenic tumours. Since its initial description, a number of studies have focused on different aspects of this lesion, attempting to explain its distinctive biological behavior. Patients and methods: a retrospective immunohistochemical studies of the expression of PCNA, Ki67 and p53 protein in 40 patients treated for OKC. Results: in the OKCs, the positivity of these markers was expressed mostly in the suprabasal layers of cystic epithelium. There is no statistically significant difference of expression between the different clinical forms (sporadic, recurrent and syndromic OKC), neither a correlation between the expression of this markers and recurrence. Conclusion: PCNA, P53 and Ki67 can not be considered as prognostic factors of OKC.

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