The Pananjung Pangandaran Strict Nature Reserve (PPSNR) is one of the sanctuary reserve area in the southern of Java, Indonesia that has unique of plants, animals, and ecosystem. However, the location of the nature reserve area that adjacent to the nature recreation parks has lead to the disturbance of natural resources due to human interferences. This research aims to assess the distribution of human activities in PPSNR and identify factors affecting those activities. The behavioral mapping method was carried out based on interviews with 209 respondents selected by an accidental sampling technique during two weeks field survey. In addition, a direct mapping method using GPS handheld was carried out to collect coordinates data of human activities and their movements. The results showed that human activities in PPSNR could be grouped into tourism activities and non-tourism activities. It was observed that distance from visitors’ house, type of attraction, and time of activity were the main factors affecting human activities in PPSNR. Finally, the authorities must take measures to control human activities in PPSNR, otherwise their impacts on the natural resources will be worsen.