No abstract

Forty-two winter wheat varieties and 193 F2 and BC,F2 seeds were screened for Glu-Dld allele encoding the HMW glutenin subunits 5 + 10 using polymerase chain reaction (PCR). The segregating populations originated from crosses involving wheat parents with good and poor bread-making quality, A clear PCR product of 450 bp, representing lDx5 of the Glu-Dld allele was identified in 24 varieties and 111 hybrid seeds. Four different Glu-Dl alleles: a (2 + 12), b (3 + 12), c (4 + 12) and d (5 + 10) were detected using sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE), Only genotypes possessing Glu-Dld gave a positive PCR signal, hexaploid triticale and 4 x wheat lacking Glu-Dl locus gave a negative signal. The efficiency of PCR selection for bread-making quality in early generations using half seed is discussed.