A field experiment was conducted during kharif season of 2021, at crop research farm of Department of Agronomy at Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj in North Eastern plains of Eastern Uttar Pradesh with the objective to study the effect of bio fertilizer and spacing on growth, yield and economics of Pearl millet (Pennisetum glaucum L.) Var. VARUN - 666 under Randomized block design comprising of 9 treatments of which treatments (T1-T9) with different bio fertilizer combination of Azatobacter and Azospirillum along with spacing which are replicated thrice. The experimental results revealed that Application of Azotobacter @ 12.5g/kg +Azospirillum @ 12.5g/kg + 60 cm ×15 cm recorded significantly higher plant height (210.20 cm), Number of leaves/hill (40.3), Highest plant dry weight (49.49 gm), number of tillers/hill (3.3), number of ears/hill (2.43), number of grains/ear head (2097.33), grain yield (2.77 t/ha), straw yield (3.91 t/ha net return (Rs.1,03,453/ha), gross return (Rs.1,41,440/ha) and benefit: cost ratio (2.72).