To obtain carnation variants differing from those produced by organogenesis alone, in vitro petal cultures were subjected to gamma irradiation. Histological analysis revealed the surface origin of buds and the different steps in meristem formation. A dose of 40 Gy administered on the fourth day of culture produced variants of horticultural interest in 'Niky'. This period corresponded to dedifferentiation of cells that subsequently developed into buds.Abbreviations: BA -benzyladenine, NAA -naphthaleneacetic acid

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