In order to study the effect of phosphate biofertilizer and chemical fertilizers of nitrogen, phosphorus and sulfur on some growth and yield components of Camelina sativa L., a study was conducted based on a factorial experiment; this was done in a randomized complete block design with three replications at Kazeroon during the 2016 growing season. Treatments included bio-based phosphorus (P) fertilizers (Barvar-2) containing phosphate solubilizing bacteria (PSB) (the Pseudomonas putida Strain 13P and Strain 5P and the Pantoea agglomerans Strain 5P) as the first factor at two levels (inoculated and uninoculated seeds) and chemical fertilizer as the second factor, including control, nitrogen, phosphorus and sulfur alone, based on the recommended doses of NPS and 50% recommended NPS fertilizers. The results showed that the effect of the use of the biofertilizer on plant height and number of seeds per silique and the number of siliques per plant, 1000 seed weight and harvest index were significant, but the number of branches, grain yield and biological yield were not significant. The use of chemical fertilizer as well as the interaction of chemical fertilizers × phosphate biofertilizer on all traits was significant. The combined use of bio-inoculants and chemical nitrogen + phosphorus + sulfur fertilizer, as compared to the control, increased plant height by 50.58%, the number of branches by 26.7%, seed number per silique by 29.8%, the grain yield by 86.88%, biologic yield by 63.58%, and harvest index by 32.36%. Nitrogen consumption increased the number of siliques per plant by 27.84% and nitrogen + sulfur treatment imrpoved 1000 seed weight by 54.54%, relative to the control treatment. So, according to the results of this experiment; the best fertilizer treatment for Camelina sativa L. production was the combination of nitrogen + phosphorus + sulfur fertilizer plus biofertilizer application.