Binocular disparity, the difference between left and right eye images, is a powerful cue for depth perception. Many neurons in the visual cortex of higher mammals are sensitive to binocular disparity, with distinct disparity tuning properties across primary and higher visual areas. Mouse primary visual cortex (V1) has been shown to contain disparity-tuned neurons, but it is unknown how these signals are processed beyond V1. We find that disparity signals are prominent in higher areas of mouse visual cortex. Preferred disparities markedly differ among visual areas, with area RL encoding visual stimuli very close to the mouse. Moreover, disparity preference is systematically related to visual field elevation, such that neurons with receptive fields in the lower visual field are overall tuned to near disparities, likely reflecting an adaptation to natural image statistics.Our results reveal ecologically relevant areal specializations for binocular disparity processing across mouse visual cortex.Keywords mouse visual cortex, binocular disparity, two-photon calcium imaging, optical imaging, higher visual areas, random dot stereogram, visual depth processing understood. Apart from V1, areas LM and RL contain the largest representation of the binocular visual field (Garrett et al. 2014; Zhuang et al. 2017), making them candidate areas for investigating downstream processing of binocular disparity in mouse visual cortex. In turn, comparison of disparity tuning across different mouse visual areas might help delineating their functional specializations.
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