No abstract

The rotifer, Brachionus plicatilis, pre-fed on baker's yeast, was enriched for 6 h with three commercial products from Artemia Systems S.A., namely Protein Selco (microcapsules), Dry Selco (microparticles) and Super Selco (an emulsion containing high levels of n-3 HUFA). The biochemical composition (protein, carbohydrate, total lipid, lipid classes and fatty acids) and dry weight of the rotifers before and after the enrichment experiments were studied. Two of the enrichers, Dry Selco and Super Selco, are rich in lipid but poor in protein and carbohydrate. Protein Selco contains lipid as well as protein and carbohydrate. The biochemical composition and the dry weight of the rotifers were changed after 6 h of enrichment. The percentage of protein and lipid increased with all of the three enrichers and the percentage of carbohydrate decreased slightly. Rotifer dry weight increased in rotifers enriched on Protein Selco. Phospholipids, sterol esters+ waxes, triacylglycerols and free fatty acids increased after the enrichment with the three enrichers while sterols slightly decreased when the rotifers were fed on Protein Selco. The fatty acid content of the rotifers increased after the enrichment with all of the enrichers and this increase was particularly apparent in the case of n-3 HUFAs (20:5n-3 and 22:6n-3).

Abstract. Ostrea edulis larvae from an adult stock maintained in a raft in the Ria of Arousa (Galicia, Spain), were cultured in 1988 under four different food regimes: Pavlova lutheri, Isochrysis galbana, Phaeodactylum tricornutum and Skeletonema costatum. Growth, mortality, percentage of eyed larvae in each tank, as well as the biochemical composition of larvae at different stages of development were also studied. Protein and carbohydrate levels increase during larval development. Total lipids also increase from liberation to Day 8, and increase slightly or decrease from Day 8 to the beginning of metamorphosis. Since lipids are the main energy reserve in larvae, it is very plausible that they are being consumed during development, especially when metamorphosis begins. Data on growth, survival and biochemical composition of these larvae, which spawned 4 d after transfer of parental stock to the conditioning circuit, are compared with unpublished data on larvae from a laboratory conditioned broodstock.

This is a single-center translational research study that aimed to look for key targets involved in Crohn’s disease and define molecular pathways through different functional analysis strategies. With this approach, we have identified and described a novel target, the almost unknown TMIGD1 gene, which may be key in the recovery of injured mucosa involving intestinal epithelial cell differentiation.

Monthly ichthyoplankton cruises were made over 3 annual cycles in the Ria of Vigo (Galician coasts, NW Spain). A total of 43395 teleostean eggs belonging to 14 farmlies and 7058 larvae of 21 families were collected. The largest densities of eggs were found during winter and spring, whereas the greatest numbers of larvae were collected during spring and summer. Eggs and larvae were found all over the na, although most of the species were most abundant in the outer area of the ria. The Ria of Vigo appears to be a malor spawning ground for many of the species recorded.