The free amino acids as well as the total amino acid content of Russian corn steep liquor (CSL), French CSL, British CSL, Italian CSL, German dried version of CSL, and Egyptian CSL were identified. The free amino acids and those obtained after the acid hydrolysis in each sample were almost the same. Alanine was the predominant free amino acid found. The major amino acids were arginine, aspartic acid, histidine, glutamic acid, glycine, leucine, lysine, serine and valine with the exception of Italian CSL and German dried CSL, while phenylalanine, methionine, tyrosine, proline as well as cystine were present in lesser amounts. The relation between the amino acid constituents of the different brands of CSL and their penicillin activity were discussed.
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