Aim. The short arm of 1R rye (Secale cereale L.) chromosome is widely used in the breeding of bread wheat (Triticum aestivum L.), in particular 1RS.1BL, to introsgress genes of resistance to leaf (Lr26), stem (Sr31), striped (Yr9) rusts, as well as powdery mildew (Pm8); 1RS.1AL carries Gb2/Gb6 resistance genes to the wheat aphid (Schizaphis graminum Rondani), powdery mildew (Pm17), and the Cmc4 resistance gene to the Aceria tosichella Koifer mite, which is a vector for spreading of wheat mosaic virus. The aim of the research is to reveal molecular genetic polymorphisms of short arm rye 1RS chromosomes of different origins in bread wheat lines with 1RS.1BL translocation or 1R(1B) substitution from different sources. Methods. Genetic polymorphism of lines was analyzed by using PCR with a number of rye and wheat microsatellite markers. Results. It was shown that the CWXs line has a recombinant 1RS arm that contains the chromosomes parts of 1RS of the parental lines H242/97-2 and H273/97, due to crossover event, which led to the recombination of marked loci. Conclusions. Molecular genetic polymorphism has been reviled in 1RS.1BL translocations and 1R substituted rye chromosomes of different origins in H242/97-2, CWXs, H273/97, PavonMA1, Salmon lines, as there are different alleles present at loci: Xscm9, Xtsm422, Xgwm752, Xgwm18, Taglgap. Keywords: polymorphism, 1RS.1BL translocation, PCR analysis, microsatellites markers.

The aim of the work was to determine alleles of Ppd-A1, Ppd-B1, Ppd-D1, Rht8c, Rht-B1, Rht-D1, Pina-D1, Pinb D1, Wx-A1, Wx-B1, Wx-D1 genes of wheat varieties from the Poltava State Agrarian Academy (PSAA). Methods. DNA extraction, allele-specific and SSR-PCR, electrophoresis in agarose and polyacrilamide gels. Results. The vast majority of varieties (9) can be characterized as Ppd-A1b, Ppd-B1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1a, Pina-D1a, Pinb-D1b, Wx-A1a, Wx-B1a, Wx-D1a. Levada, Tsarychanka, Lyutenka and Orzhytsia varieties were carriers of Ppd-A1b, Ppd-B1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1b, Pina-D1a, Pinb D1b, Wx-A1a, Wx-B1a, Wx-D1a alleles, and the cultivar Sydor Kovpak — Ppd-A1b, Ppd-B1b, Ppd-D1a, Rht8c, Rht-B1a, Rht-D1a, Pina-D1a, Pinb-D1A/b, Wx-A1a, Wx-B1a/b, Wx-D1a — was heterogeneous by genes, which are responsible for the quality of the grain. Conclusions. PSAA winter wheat varieties are characterized by a high degree of homogenity in the alleles of genes that determine important economic and agronomical traits. This fact could be explained by the needs of a certain growing zone and traditional breeding approaches of wheat in the PSAA.Keywords: Allele-specific markers; winter bread wheat; dwarfing genes; sensitivity to photoperiod; Wx; Pina and Pinb.