The flavonoid apigenin was isolated from aerial part of P. oleracea L. The dried sample of plant was powdered and subjected to soxhlet extractor by adding 80 mL of ethanol : water (70 : 30). The extract was centrifuged at 11000 rpm for 30 min; supernatant was taken for further use. The fraction was concentrated and subjected to PTLC. The R f value of isolated apigenin was calculated (0.82). Purified material was also subjected to its IR spectra, LC-MS, NMR, and HPLC for structural elucidation. The apigenin so-obtained was subjected to antibacterial activity on five pathogenic bacterial strains like Pseudomonas aeruginosa, Salmonella typhimurium, Proteus mirabilis, Klebsiella pneumoniae and Enterobacter aerogenes; among all the bacterial strains, Salmonella typhimurium (17.36 ± 0.18) and Proteus mirabilis (19.12 ± 0.01) have shown maximum diameter of inhibition zone for flavonoid and remaining bacterial strains have shown moderate diameter of inhibition zone when compared with control values 14.56 ± 0.21 and 11.68 ± 0.13, respectively. The minimum inhibitory concentration (MIC) of the flavonoid isolated from P. oleracea L. was tested at the concentration ranging from undiluted sample to 10 mg per mL of concentration. The minimum inhibition concentration (MIC) for the flavonoid for all tested bacterial strains was found to be >4 mg per mL. Hence, the apigenin has antibacterial property and can be used to develop antibacterial drugs.

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ABSTRACT. Preliminary phytochemical screening of various extracts of Amoora ruhituka bark were investigated which reveals the presence of several secondary metabolites in each extracts. The antibacterial activity of all the extracts was tested against four gram negative bacterial strains. The results indicated the zone of inhibition which ranges from 11.30±577 to 18.7±0.577 for different extracts in which Methanol extract has shown highest zone of inhibition for Salmonella typhimurium followed by Enterobacter aerogenes , E coli and P aeruginosa whereas benzene extract has showed the least zone of inhibition and the minimum inhibitory concentration (MIC) of the different extracts ranging from 0.78 mg/ml to 6.25mg/ml.The complete results of this study provides a essential data for the use of Amoora ruhituka for the treatment of infection associated diseases

Preliminary phytochemical screening of various extracts of Amoora ruhituka bark were investigated which reveals the presence of several secondary metabolites in each extracts. The antibacterial activity of all the extracts was tested against four gram negative bacterial strains. The results indicated the zone of inhibition which ranges from 11.30±577 to 18.7±0.577 for different extracts in which Methanol extract has shown highest zone of inhibition for Salmonella typhimurium followed by Enterobacter aerogenes , E coli and P aeruginosa whereas benzene extract has showed the least zone of inhibition and the minimum inhibitory concentration (MIC) of the different extracts ranging from 0.78 mg/ml to 6.25mg/ml.The complete results of this study provides a essential data for the use of Amoora ruhituka for the treatment of infection associated diseases

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