A total 1006 animals (300 cattle, 300 buffaloes, and 300 sheep and106 goats) were selected from private farms and suspected to suffer from brucellosis from different localities in Gharbiya governorate, as well as 50 rats (31Rattus rattus &19 Rattus norvegicus) and 15 stray dogs were collected from the same localities associated with examined animals. In addition, 160 persons suffering from fever suspected to be brucellosis were collected (80 workers contact with examined animals and 40 from fever hospitals). Serological tests were carried out by using Rose Bengal plate (RBPT), Buffered Acidified plate test (BAPAT), Complement Fixation test (CFT), Tube Agglutination test (TAT) and 2-Mercapto-Ethanol test (2-MET). The results showed that the percentage of positive reactors were 9%, 7.3%, 9.3% 8.5%, 8% and 0% using RBPT in cows, buffaloes, sheep, goats, rats and dogs respectively. Meanwhile the percentage of positive reactors using BAPAT was 9.6%, 8.3%, 10.7%, and 9.6% in cows, buffaloes, sheep and goats and by using CFT the percentage was 9.3%, 8%, 10.3% and 10.3% in previously examined animals. Also the result of TAT was 8% in rats and 0.0% in dogs. The occurrence of brucellosis in sheep and goats was higher than cows and buffaloes. Finally, the results in humans were 13.1%, 11.3% and 10% by using RBPT, TAT and 2MET respectively. The incidence of brucellosis was higher in males (14.9%) than females (3.8%) and higher in humans aged between 20-30 years.

A total of 127 specimens (13 aborted foeti, 46 milk samples, 37 lymph nodes, 14 livers, 14 spleen and 6 vaginal discharges) were collected and examined for isolation and typing of Brucella microorganism. The results detected 15 strains (5 aborted foeti, 4 milk, 5 lymph nodes and 1 spleen) were detected and typed as Br. melitensis biovar 3. Application of PCR test for rapid identification of Brucella strains which isolated from lymph nodes five of naturally infected animals (two cattle, one buffaloes, one sheep and one goat) revealed that all samples were reacted positively with Br. melitensis specific DNA products with a molecular size of 731 pb. On sequencing, the Nucleotide sequence alignment of obtained sequences with other Brucella strain indicated that the obtained isolate have high identity with Br. melitensis biovar 3. The bacteriocidal activity of tested disinfectants against isolated Br. melitensis strain at variables concentration revealed that halogen showed highest bactericidal activity followed by QACs and phenolic while alkaline wasthe lowest effect.