It is essential for collection of blood samples from laboratory animals employed in wide range of scientific research. There is a no. of techniques available for blood sample collection from Rats/mice. The stress produced in animals during the blood collection may compromise with the research data which may invalidate the results. It is necessary that the blood sample collection is done by trained individuals so as to minimize the stress and other physiological reactions in the animals. This article reviews some of the common approved blood collection techniques for rats/mice.

Objectives:To formulate a polyherbal formulation and evaluate its antiarthritic activity against Freund's complete adjuvant induced arthritis in Female Wistar rats.Materials and Methods:Glycosmis pentaphylla, Tridax procumbens, and Mangifera indica are well-known plants available throughout India and they are commonly used for the treatment of various diseases including arthritis. The polyherbal formulation was formulated using the ethanol extracts of the stem bark of G. pentaphylla, whole plant of T. procumbens, and leaves of M. indica. The polyherbal formulation contains the ethanol extracts of G. pentaphylla, T. procumbens, and M. indica in the ratio of 2:2:1. The quality of the finished product was evaluated as per the World Health Organization's guidelines for the quality control of herbal materials. Arthritis was induced in female Wistar rats using Freund's complete adjuvant (FCA), and the antiarthritic effect of polyherbal formulation was studied at doses of 250 and 500 mg/kg. The effects were compared with those of indomethacin (10 mg/kg). At the end of the study, blood samples were collected for biochemical and hematological analysis. The radiological examination was carried out before terminating the study.Results:Polyherbal formulation showed significant antiarthritic activity at 250 and 500 mg/kg, respectively, and this effect was comparable with that of indomethacin. The antiarthritic activity of polyherbal formulation is supported by biochemical and hematological analysis.Conclusion:The polyherbal formulation showed signinicant antiarthritic activity against FCA-induced arthritis in female Wistar rats.