ABSTRACT:The occurrence of Eriophyes tiliae (Pgst.) subspecies was observed in lime trees in the urban area of Brno. Mites presence was assessed in relation to the lime species, its age and health conditions. Analysis of the occurrence of liosoma, tiliae and nervalis subspecies was carried out on leaves of 3,245 lime trees from the viewpoint of the host preference and spatial distribution of attack in the area of the city and potential effects of air pollution. Subspecies liosoma and tiliae occurred in 13% of lime trees, the subspecies of nervalis was found only in 4 limes. Eudominant subspecies of E. tiliae preferred mature trees and young trees reaching height of mature trees in good health conditions. The distribution of Eriophyes tiliae on lime trees in the area of Brno depended on the distribution of suitable host trees and was not affected by air pollution.