We studied biochemical, morphological, and histological parameters of the extracellular matrix in scaffold-free tissue engineering chondrotransplants prepared from chondrocytes isolated from knee joint cartilage biopsy specimens of Haflinger horses (age 3.5-14 years) and in transplants prepared on the basis of commercial matrixes of Ethisorb® and Chondro-Gide(®). A total of 50 tissue-engineering constructs were designed and analyzed. Passage 2 cells populations were used. Mechanical stimulation during culturing of scaffold-free constructs considerably activated synthesis of the basic components of the cartilage matrix (proteoglycan concentration was 35% of the content in the native tissue, and the content of collagen-specific amino acids hydroxyproline and hydroxylysine attained 29.3 and 12.7%, respectively). The architecture of these cartilage constructs was morphologically and histologically similar to the native cartilage tissue. Insufficient support of the chondrogenesis by scaffold-based chondrotransplants and no differences between these constructs by the studied parameters were noted despite different chemical nature and structure of these scaffolds.

No abstract

The studies were conducted on groups of fattening pigs aged 3 to 5.5 months, which were kept in four sectors with different air cooling systems. For observation we used the animals assessment technique presented in a Welfare assessment protocol for pigs. It is revealed that the indoor air cooling method at temperatures in the control sector ranged from 19 to 26.6 o C does not have a decisive influence on the suite of welfare parameters of fattening pigs (Fatting young pigs. in spring and summer periods closing parenthesis. Cooling method based on air inflow through underground tunnels provides a good level of ventilation, reduces the concentration of harmful gases, and ensures lower humidity as compared to control. At that, it reduces the incidence of cannibalism in pigs (P<0.05). The reduction of animal fouling in sectors with a high content of harmful gases with some reduction in temperature (by 0.8-1.5°C) and increase in relative humidity (by 1.3-4.3%) is possibly due to the inhibition of general activity of animals. The authors revealed also the effect of animal feeding (wet and dry feeding systems) on the animal fouling intensity (P<0.05). The incidence of cannibalism in pigs was decreased in the farm with dry feeding system (P<0.05).

Учредитель и издатель: Founder and editor: ФГБОУ ВО «Казанская государственная академия ветеринарной медицины имени Н.Э. Баумана» (ФГБОУ ВО Казанская ГАВМ) FSBEI HE «Kazan Bauman State Academy of Veterinary Medicine»(FSBEI HE KSAVM) Печатается по решению редакционной коллегии Казанской государственной академии ветеринарной медицины им. Н.Э. Баумана от 5 Июня 2019 г

No abstract