Peanuts (Arachis hypogaea L.) are known to be a source of several important B-vitamins, including niacin (Vitamin B3) Florunner and 6% in NC7 (p50.0128). Mean niacin concentrations in the Core of the Core samples were found to range from 13.9 to 20.7 mg/100g DW. Levels of niacin in the Core samples tested were found to be higher than Florunner (12.2 mg/100 DW) and NC7 samples (13.8 mg/100g DW) grown at the same location and in the same year. Significant differences in niacin content among NC7 and Florunner UPPT entries across 10 locations indicated a potential for genetic variation that is potentially exploitable by breeders. This is supported by the significant differences found among the Core of the Core entries and NC7 and Florunner samples from the same location and year.