The resident oral microbiota plays an important role as a factor in its colonization resistance that ensures human oral health. However, at the same time, oral microorganisms can act as causative agents of infectious diseases. Scientists emphasize that in the situation when bacteria demonstrated increased resistance to antibiotics, quorum sensing is a particularly attractive target, since this mechanism controls several factors of bacterial virulence that affect adhesion, invasion and colonization of microorganisms during the development of the pathological process. Quorum sensing inhibitors can be enzymes and various chemicals that able to affect quorum sensing. Streptococcus mitis is a representative of the resident microbiota of the human oropharynx. But despite its commensalism, Streptococcus mitis in association with other microorganisms takes part in many pathological processes. Objective of this study is to study the effect of Dekasan and Iodoform antiseptics on the adhesive properties of Streptococus mitis clinical isolates taken from the oral cavity of patients with infectious and inflammatory post-extraction complications. Materials and methods: examined clinical isolates of Streptococus mitis, which were isolated from 8 patients undergoing treatment for infectious-inflammatory post-extraction complications in the medical and surgical department of Poltava Regional Centre of Stomatology - Dental Clinical Polyclinic. Identification of selected cultures was carried out using an automatic bacteriological analyzer Vitec-2 compact bioMarieux (France). We used the average values of subbacteriostatic concentrations of the active ingredients of antiseptics. Adhesive properties of clinical isolates under the influence of Dekasan and Iodoform were determined according to the method of V.I. Brylis. Conclusions: antiseptics have different effects on the adhesive properties of clinical isolates of Streptococus mitis.

Antibiotic resistance is a global challenge, whose emergence is associated with the irrational and uncontrolled use of antimicrobial drugs, or the use of antibiotics to prevent local and systemic complications during the surgical operations. Therefore, the number of failed outcomes of the planned treatment of infectious and inflammatory processes with antibiotics has increased. Given this fact, it is important to study the potential of antiseptic drugs, which usually do not cause the microorganism resistance and thus are more advantageous over antibiotics. Objective of this study is to compare the effects of antiseptics Decasan, Chlorhexidine and Iodoform on clinical and typical strains of microorganisms using the disco-diffusion method according to the EUCAST. Materials and methods: Typical strains of Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 14990 were used as the studied cultures of microorganisms. Clinical isolates of the microorganisms Staphylococcus aureus, Staphylococcus epidermidis were collected from patients with postextraction complications, who took treatment at surgical departments of Poltava. Identification of isolated cultures was performed using an automatic bacteriological analyzer Vitec-2 compact bioMarieux (France). The antibacterial effect of antiseptic drugs Dekasan, Chlorhexidine, Iodoform was studied. Given that iodoform is poorly soluble in water, we used its 5% alcohol solution, which was prepared ex tempore. The susceptibility of the studied strains to antiseptics was determined by disco-diffusion method according to the EUCAST committee. The determination was performed by using Mueller-Hinton medium (HIMEDIA, India) with standard sterile disks, which were not impregnated (HIMEDIA, India). Then we impregnated them with solutions of Decasan, Chlorhexidine and 5% alcohol solution of iodoform. We used a microbial inoculum equivalent to according to the McFarland turbidity standard. Prepared Petri dishes containing impregnated paper disks were incubated overnight at 35-36 °C, after which the results were determined. Results and discussion: The study has shown that the antiseptic Decasan has a greater antistaphylococcal effect on typical strains and clinical isolates of staphylococci compared to other antiseptics, which are often used in dentistry. The lowest antistaphylococcal effect on the studied microorganisms has been found by applying 5% alcohol solution of iodoform, Chlorhexidine has demonstrated the moderate effect on the strains under the study.

According to the literature, microorganisms of the genus Kocuria are becoming widespread, but not as avirulent representatives of the normal microbiota of the human body, but as pathogens. Recently, after the identification of the causative agents of infectious and inflammatory processes, various types of Kocuria have been included in the list of etiological agents isolated from patients with cholecystitis, endocarditis, peritonitis, and meningitis as well as from carious tooth cavities and post-extraction tooth sockets. Bacteria of the genus Kocuria possess pathogenicity that enables them to play an important role in the occurrence of infectious and inflammatory processes in various organs and systems of the human body. Given that antibiotics are the most commonly used drugs of choice in medical and dental practice for the treatment of infections and inflammations, antibiotic resistance is considered as a global health and development threat. In order to overcome the challenge of antibiotic resistance, antiseptics, a group of medicines with a pronounced antimicrobial effect, are being increasingly used for the treatment of infectious and inflammatory processes. The purpose of this study is to investigate the antibacterial and antiadhesive activity of dekasan, and drugs of comparison, chlorhexidine and iodoform, against clinical isolates of Kocuria spp. isolated from the oral cavity of patients with infectious inflammatory post-extraction complications. Materials and methods: five clinical isolates of Kocuria spp. were studied as microorganisms isolated from patients, who received the treatment for infectious and inflammatory post-extraction complications at the medical and surgical departments of Poltava Regional Center of Stomatology – Dental Polyclinic. We determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBcC) of antiseptics against clinical isolates of Kocuria spp. taking into account ISO recommendations. The adhesive properties of clinical isolates of Kocuria spp. were assessed by exposing them to subbacteriostatic concentrations of antiseptics, namely dekasan, chlorhexidine, and iodoform. The evaluation was conducted using erythrocytes of blood group 1(0) Rh+ and considering the index of microorganism adhesion (IAM) following the method developed by V. I. Brillis. The results obtained have demonstrated variations in the antimicrobial and anti-adhesive activities among the tested antiseptics, dekasan, chlorhexidine, and iodoform.