Conjoined twins result from an abnormal process in the development of monozygotic twins. The incidence of conjoined twins ranges from 1 in 50,000 to 1 in 100,000 pregnancies [1]. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) have increased the frequency of multiple pregnancies [2]. The incidence of monozygotic twins is generally constant at one in 250 pregnancies [3]. There were two reports of co njoine d t wi ns w hich occur red af ter assist ed reproductive technology. One case occurred after a combination of IVF and assisted hatching [4]. The other o c c u r r e d a f t e r a c o m b i n a t i o n o f I C S I a n d cryopreservation of embryos [5].In our patient, we performed ICSI and cryopreserved the embryos obtained. Subsequently, we thawed 2 embryos in a natural cycle. After performing assisted hatching, we transferred the embryos. This resulted in a pregnancy, which was determined to be a triplet pregnancy by transvaginal ultrasonography performed a t w e ek 1 0 w e eks ' g est a ti on . A n d a t h or ac oomphalopagus as a conjoined twin was diagnosed in two fetuses.
Case ReportA 30-year old gravida 0, para 0 woman underwent her first ICSI with testicular sperm extraction (TESE) in a case involving obstructive azoospermia in the husband. Since spermatozoa could not be obtained from the epidid ymis, TE SE was perf ormed, follow ed by cryopreservation of testicular spermatozoa. From the first day of the treatment cycle, 900 µg of GnRHa (Suprecur, Hoechst) was administered daily (short protocol). Stimulati on was admi nistered in the treatment of menstrual cycles with pure FSH and hMG, with a 10,000 IU dose of hCG administered when the maximum diameter of follicles reached 20 mm. The oocytes were collected 35 hours thereafter. Five oocytes, all grade 1, according to Veeck's classification [ 6 ] , w e r e r e t r i e v e d . I C S I w i t h m o t i l e t h a w e d spermatozoa was performed for all oocytes. All oocytes were successfully fertilized. Three of the 5 oocytes were transferred to the uterus during the treatment cycle. The oocytes, all were grade 1, were transferred at the 7 to 8 cell stage three days after oocyte retrieval. Two oocytes out of 5 were cryopreserved at the 4 cell stage by the slow-freeze method. Finally, none of the transferred oocytes resulted in pregnancy in the first
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