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The study of the relationships between the yield components can help to understand the physiological basis of crop yields. This research was conducted to investigate the genetic diversity of 168 Iranian wheat populations from the aspect of morpho-physiological characteristics in the Grain and Plant Improvement Institute research center in Karaj. In order to calculate the error and correct the collected data, four wheat cultivars of Sivand, Pishtaz, Pishgam, and Sirvan with three replications were used as control groups. The control group was cultivated based on augmented design and genotypes were placed in separate blocks among the control groups. The treatments and control groups were divided into three blocks and each block containing 60 plots which were planted on one-meter beds and the spacing between each plot from other plot was considered 62 cm. The necessary measurements and samples of the movement were collected systematically and by observationally method and sampling from the statistical population of each plot. Data analysis, statistical analyzes of multivariate data including cluster analysis based on multivariate analysis such as principal component analysis (PCA) and simple correlation analysis between characteristics (Pearson) are done using statistical software SPSS and SAS.