SUMMARY :The non-pathogenic beta-haemolytic streptococci from milk, forming Lancefield's group E, were physiologically defined as Streptococcus infrequens and S. subacidus. They were found to fall into four serological types. Fractionation with ethanol showed that both group-and type-specific fractions of these organisms are of polysaccharide nature which probably accounts for the success in using formamide extracts for the type-precipitin reactions. S. subacidus possesses two type antigens, only one of which is also found in S . infrequens.

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phate were incubated at 37 C. At time intervals, samples of the incubation mixture were acidified, centrifuged, neutralized to pH 7.0 with dilute NaOH, and assayed for glucose-6-phosphate and fructose-6-phosphate (Racker and Schroeder,