A novel vic-dioxime ligand containing the thiourea group, (4E,5E)-1,3-bis{4-[(4-methylphenylamino)methyl] phenyl}-2-thiooxaimidazoline-4,5-dione dioxime, (4) mmdH 2 has been prepared from N,N¢-bis{4-[(4-methylphenylamino)methyl]phenyl}thiourea, (3) mft and cyanogen di-N-oxide. Mononuclear [M(mmdH) 2 ], where M=Ni II , Co II and Cu II complexes of the (4) mmdH 2 bidentate ligand have been obtained with a 1:2 metal:ligand ratio, as do most the vic-dioximes. The complexes are formed by coordination of N, N atoms of the ligand. The vic-dioxime ligand and its some transition metal complexes have been characterized by elemental analyses, molar conductance data, magnetic susceptibility, i.r., 1 H-n.m.r and u.v.-vis. spectroscopy. Conductivity measurements have shown that mononuclear complexes are non-electrolytes. In addition, the ligands and metal complexes were screened for antibacterial and antifungal activities by agar well diffusion techniques using DMF as solvent.