Mangoes are consumed for 572,000 metric tons per year that leads to 57,200 metric tons of mango seeds that need to be eliminated. In Egypt and India, there are many researches about composition in mango seed kernel and their activities. They have found that there are fatty acids and phenolic compounds in mango seed extracted 1. These composition could be used in many industries such as food preservation, cosmetology and food ingredients 2,3. Typically, mango seed extraction uses methanol and ethanol as solvent extraction 4. These solvents cause toxic in extracted substances and resulting in pollutions. Supercritical carbon dioxide extraction has been proposed as nonsolvent residues and better retention of aromatic compounds as an alternative technique to conventional solvent extraction and mechanical pressing for extracting essentential oils 5-7 , seed oil 8 and carotenoids 9 from various plant materials. Extracted volatile oil from Aquilaria subnitegra by using supercritical carbon dioxide and hydrodistillation. The maximum yield was reached at 4.43 % from supercritical carbon dioxide at 25 MPa, 50 °C and 3 g/min of carbodioxide flow rate. Whereas the yield from hydrodistillation process was only 0.23 % 10. Moreover, extracted patchouli oil by using steam distillation and supercritical carbon dioxide. They have found that maximum yield of patchouli oil was reached 5.07 % at 14 MPa and 40 °C of supercritical carbon dioxide extraction that more than steam distillation method 11. So, supercritical fluid could be used to extract herbs and it could extract oil at