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We showed that the percentage of sturgeon oocytes ovulating in vitro in Ringer solution modified for sturgeons (RMS) considerably depends on the concentration of sodium bicarbonate and the concentra tion of progesterone. Under optimal conditions (0.5 g/L of sodium bicarbonate and 30 ng/mL of progester one), it can be higher than 80. Oocytes that matured and ovulated under such conditions are capable of nor mal development. In the best case, approximately 70% of developing embryos (of the number of ovulated oocytes) reach the stage of hatching (dead line of observation). This method of producing offspring based on the insemination of oocytes that have matured and ovulated in vitro can be used in work with single females of rare and disappearing sturgeon species.

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