Significance and Impact of the Study: Bacterial resistance development against commercial antibiotics has become one of the most significant global problems. There is a strong demand for the discovery of novel classes of antibiotics with high efficiency, low dose, no toxicity and affordable cost. According to the results described in this research, non-toxic nitrobenzylidene(quinazolin-4-yl)hydrazines (compounds 1b and 2b) can be considered promising medications for the treatment of infectious diseases owing to their proper activity against various susceptible and resistant bacteria. Furthermore, they can become industrialized because of their convenient and high-yield synthesis.