Background During April, 2021-April, 2022, the field survey was conducted in Mettupalyam Forest range of Coimbatore Forest Division which is an integral part of Nilgiris Biosphere Reserve and it was approached in a systematic random sampling method with 60 sample plots of 20x 20 m and 8 transect line survey at 0.2% sampling intensity were laid out in which study area were catalogued with 54 trees, 25 shrubs, 22 herbs and 22 grasses. Results The result revealed that the maximum dominance were observed in Atlantia monophylla (32.20), Lantana camara (43.02), Abutilon indicum (54.87) and Oplismenus burmanii (19.34%) and minimum in Glycosmis pentaphylla (12.32), Euphorbia heterophylla (2.18), Mimosa pudica (2.82) and Melinis repens (0.18%) in tree, shrubs, herbs and grass layer, respectively. Furthermore, the highest Shannon D index (H = 3.08) observed in TL, followed by GL (H = 2.76), HL (2.28) and SL (2.25) along with Simpson index also higher in GL (1-D = 0.92), TL (1-D = 0.91), HL (1-D = 0.86) and SL (1-D = 0.84), respectively. Conclusion Cataloguing flora in Mettupalayam Reserve Forest is the pioneer study which gives scientific and baseline information for conservation of flora in the forest.