Cyclic adenosine 3′, 5′‐monophosphate (cyclic AMP)‐ and cyclic guanosine 3′, 5′‐monophosphate (cyclic GMP)‐phosphodiesterase activities from rat lung were selectively inhibited by ZK 62711 and M & B 22948, respectively. Theophylline and papaverine inhibited both activities. Rat lung strips contracted by carbachol were relaxed by 4‐(3‐cyclopentyloxy‐4‐methoxyphenyl)‐2‐pyrrolidone (ZK 62711, EC25 = 7 × 10−8m) and 2‐O‐propoxyphenyl‐8‐azapurin‐6‐one (M&B 22948, EC25 = 5 × 10−7m) indicating relaxant properties of both cyclic AMP and cyclic GMP. The antigen‐induced histamine release from human basophils was inhibited by ZK 62711 (IC25 = 8 × 10−7m), whereas M&B 22948 had no effect. On the contrary, the release from rat mast cells was inhibited by M&B 22948 (IC25 = 10_6m), while ZK 62711 had no effect. These data show an inhibitory effect of cyclic AMP on histamine release to be involved with basophils, whereas cyclic GMP is predominantly involved with mast cells. It is suggested that the antianaphylactic properties of cyclic nucleotide phosphodiesterase inhibitors are mainly linked to the increase of cyclic GMP.

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A new method involving 14C‐labelled cell walls of apple leaves was developed in order to study the process of cell wall degradation in vitro and its role in pathogenesis and host‐resistance. 14C‐labelled cell walls were efficiently digested by commercial enzyme preparations and less efficiently by the polygalacturonase of Venturia inaequalis, the causal agent of apple scab. Further, degradability of cell walls from a susceptible and a resistant variety were compared, but there was no evidence for a correlation of reduced degradability with resistance. The method presented here can be regarded as a useful tool for investigations where enzymatic processes of polymerization or depolymerization of plant material is involved.